COLtest

COLtest

DISCONTINUED (REPLACEMENT: ABP-COL-T1)

INTENDED USE

Multiplate® is for research use only in the US and Canada.

Multiplate® COLtest is a reagent kit used for collagen-induced aggregation in research platelet aggregation studies on the Multiplate® analyzer. Sensitive to a cyclooxygenase inhibition, GpIIb/IIIa antagonists, and deficiencies of GpIIb/IIIa receptors.

KIT COMPOSITION

Reagents, Composition, Storage and Stability

  • MP0230: Multiplate® COLtest reagent, Kit, 3 x 1.0 ml. 150 tests. Reagent is stable until the expiration date printed on the vial label when stored at 2-8°C. Stable 7 days after reconstitution. Do not freeze the reconstituted reagent.
  • MP0093: Multiplate® COLtest Aliquot Vials, Bag, 100 color coded (brown) micro test tubes.

MEASUREMENT PRINCIPLE

The COLtest leads to platelet activation via the platelet collagen receptor. For suitable platelet activation, the release of endogenous arachidonic acid from the platelet phospholipids is necessary. Arachidonic acid is then transformed to the platelet agonist thromboxane A2 (TXA2) by the enzyme cyclooxygenase (COX-1). Cyclooxygenase is blocked by acetylsalicyclic acid (ASA) and therefore the COLtest is sensitive to the action of ASA. This mechanism also explains why the COLtest is less specific towards the action of ASA as compared to the ASPItest. In the ASPItest, a defined amount of arachidonic acid is used as the activator. However, in the COLtest, platelets are activated through endogenous TXA2 and other TXA2 independent mechanisms. Ongoing research continues to investigate this agonsist/receptor interaction.